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cd134 (ox40) antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd134 (ox40) antibody, anti-human, reafinity
    Cd134 (Ox40) Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ox40/CD134+(OX40)+Antibody%2C+anti-human%2C+REAfinity/custom%40130-126-048%4042526444
    Average 95 stars, based on 17 article reviews
    cd134 (ox40) antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Cell Isolation:

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Modification:

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Article Title: Loss of ATG5 impairs CD4+ T cell activation and promotes anti-tumor responses
    Article Snippet: After 72 hours, cells were harvested and stained with CD4-FITC (RM4-5, eBiosciences) and CD25-PE (Miltenyi) and analyzed for proliferation in a Cytoflex LX (Beckman Coulter). .. OX40- and OX40+ cells were purified using a CD4+ CD25+ regulatory T cell isolation kit for magnetic separation (130-091-041; Miltenyi Biotec) according to the manufacturer’s protocol, with the modification that the anti-OX40-PE (#119410, BioLegend) was used instead of the anti-CD25-PE provided in the kit. ..

    Staining:

    Article Title: Prognostic immunological implications of OX40L expression in the tumor microenvironment of melanoma
    Article Snippet: .. Cells were stained with fluorophore-conjugated antibodies against CD4, CD25, OX40, OX40L, and intracellular Foxp3 using the Foxp3 Buffer Set (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Cell viability was assessed using ViobilityTM dye (Miltenyi Biotec).

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy
    Article Snippet: For detection of CAR-T and CAR-NK cell counts after the coculture, CAR-T cells were labeled with 1 μM CellTrace Violet and 1 μM CellTrace FarRed according to the manufacturer’s instructions, respectively (Thermo Fisher Scientific, Waltham, MA, USA). .. For evaluation of CAR-T and CAR-NK cell activation following coculture with tumor cells and MV, staining with CD3 (CD3 antibody, VioBlue, anti-human, REAfinity), CD16 (CD16 antibody, anti-human, PerCP-Vio700, REAfinity), OX40 (CD134 antibody, PerCP-Vio700, anti-human, REAfinity), NKG2D (CD314 antibody, PE, anti-human, REAfinity), DNAM-1 (CD226 antibody, PE-Vio770, anti-human, REAfinity), 2B4 (CD244 antibody, APC, anti-human, REAfinity), CD48 (CD48 Antibody, APC-Vio770, anti-human, REAfinity) (all Miltenyi Biotec, Bergisch Gladbach, Germany), and OX40L (CD252 antibody, BV421, anti-human) (Becton Dickinson [BD], New Jersey, USA) was carried out after 72 h of coculture. ..

    Activation Assay:

    Article Title: Enhanced solid tumor cell targeting by a neoepitope-encoding oncolytic measles virus combined with CAR therapy
    Article Snippet: For detection of CAR-T and CAR-NK cell counts after the coculture, CAR-T cells were labeled with 1 μM CellTrace Violet and 1 μM CellTrace FarRed according to the manufacturer’s instructions, respectively (Thermo Fisher Scientific, Waltham, MA, USA). .. For evaluation of CAR-T and CAR-NK cell activation following coculture with tumor cells and MV, staining with CD3 (CD3 antibody, VioBlue, anti-human, REAfinity), CD16 (CD16 antibody, anti-human, PerCP-Vio700, REAfinity), OX40 (CD134 antibody, PerCP-Vio700, anti-human, REAfinity), NKG2D (CD314 antibody, PE, anti-human, REAfinity), DNAM-1 (CD226 antibody, PE-Vio770, anti-human, REAfinity), 2B4 (CD244 antibody, APC, anti-human, REAfinity), CD48 (CD48 Antibody, APC-Vio770, anti-human, REAfinity) (all Miltenyi Biotec, Bergisch Gladbach, Germany), and OX40L (CD252 antibody, BV421, anti-human) (Becton Dickinson [BD], New Jersey, USA) was carried out after 72 h of coculture. ..

    Isolation:

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. CD8 + T cells were magnetically isolated from spleens (after four treatments) and tumor tissues (after three treatments) of Control-, OX40-, MPLA+IFNγ-, or Combo-treated mice using CD8α (Ly-2) MicroBeads (130-117-044, Miltenyi Biotec) and co-cultured with CFSE-labeled MC38 target cells (1×10 5 cells/well) at an effector-to-target ratio of 10:1 in complete RPMI-1640 medium supplemented with 10% FBS for 36 h at 37°C. .. Cells were then harvested by trypsinization, stained with 7-AAD viability dye (1 μg/mL), and analyzed by flow cytometry (BD LSRFortessa).

    Control:

    Article Title: Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapy
    Article Snippet: .. CD8 + T cells were magnetically isolated from spleens (after four treatments) and tumor tissues (after three treatments) of Control-, OX40-, MPLA+IFNγ-, or Combo-treated mice using CD8α (Ly-2) MicroBeads (130-117-044, Miltenyi Biotec) and co-cultured with CFSE-labeled MC38 target cells (1×10 5 cells/well) at an effector-to-target ratio of 10:1 in complete RPMI-1640 medium supplemented with 10% FBS for 36 h at 37°C. .. Cells were then harvested by trypsinization, stained with 7-AAD viability dye (1 μg/mL), and analyzed by flow cytometry (BD LSRFortessa).



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